The IstanbulAirport started serving international and domestic flights on April 6 after its mega move.
Turkey's flag carrier TurkishAirlines dispatched its first flight to capital Ankara at 2 p.m. local time (1100GMT).
İlker Aycı, chairman of TurkishAirlines, said in a statement: "Today in our new home, we open the doors of a new adventure and fly to our capital once again for our first flight."
On April 5, the big switch from Ataturk Airport to the new Istanbul airport began.
The first phase of the airport with an annual capacity of 90 million passengers officially opened last October.
At full annual capacity of 200 million passengers- with the completion of all four phases with six runways until 2028- IstanbulAirport is set to become a global aviation hub with flights to over 350 destinations around the world.
After its completion, the airport will serve 250 aviation companies worldwide with daily 2,000 plane landing and taking off capacity.